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. 2018 Jun 1;22(6):919–928.e6. doi: 10.1016/j.stem.2018.05.001

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Crown Copyright © 2018 Published by Elsevier Inc. All rights reserved.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Xist Is Transiently Upregulated Monoallelically in Males and Biallelically in Females In Vivo

(A and B) RNA FISH for Xist in representative male and female epiblasts of embryos at E4.5 and E5.5 (A) and at E4.75–E5.0 (B).

(B) Examples of cells with monoallelic Xist expression in males and biallelic expression in females are delineated with yellow dashed lines. Higher magnification of these cells is displayed in the bottom panels.

(C) Percentage of cells with biallelic Xist over total number of epiblast cells expressing Xist in female embryos at indicated developmental stages. Error bars represent ± SD.

Scale bar represents 10 μm.