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. 2018 Jun 5;9(3):e00923-18. doi: 10.1128/mBio.00923-18

FIG 6 .

FIG 6 

MLV and HIV induce a DDX41-dependent IFN-β response in BMDMs and BMDCs. (A) BMDMs and BMDCs isolated from mice of the indicated genotypes were infected with MLV, and at 2 hpi, IFN-β levels were measured. The data in the graph are the averages from 3 different experiments, each with macrophages and DCs from a different mouse. NS, not significant; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001 (unpaired t test). Verification of the knockdown is shown in Fig. S5B. ELISAs measuring IFN-β protein are shown in Fig. S5A. (B) HIV pseudotype infection in cGas and Ddx41 KO BMDMs and relative infection of BMDMs and BMDCs by MLV. HIV cores pseudotyped with ecotropic MLV glycoproteins were used to infect BMDMs (left) or BMDCs (right) from mice of the indicated genotypes. Trex1 knockdowns are shown in the right panels. Shown are the averages from 3 independent experiments. ***, P ≤ 0.001; ****, P ≤ 0.0001 (unpaired t test). (C) DCs and macrophages were isolated from MLV-infected mice at 16 dpi, and levels of integrated MLV were determined by qPCR. **, P ≤ 0.01. Mock indicates mock-infected cells.