Table 2.
Comparison of three methods for detecting allergens of peanut, sesame, and soybean in a range of commercial foods.
| sample | LAMP-microfluidic chip | Typical-LAMP assay | traditional RT-PCR | sample | LAMP-microfluidic chip | Typical-LAMP assay | traditional RT-PCR |
|---|---|---|---|---|---|---|---|
| Biscuit 1 | +−− | +−− | +−− | Candy 1 | +−− | +−− | +−− |
| Biscuit 2 | +−− | +−− | +−− | Candy 2 | +−− | +−− | +−− |
| Biscuit 3 | −+− | −+− | −+− | Candy 3 | −+− | −+− | −+− |
| Biscuit 4 | −+− | −+− | −+− | Candy 4 | −+− | −+− | −+− |
| Biscuit 5 | −−+ | −−+ | −−+ | Candy 5 | −−+ | −−+ | −−+ |
| Biscuit 6 | −−+ | −−+ | −−+ | Candy 6 | −−+ | −−+ | −−+ |
| Biscuit 7 | −−− | −−− | −−− | Candy 7 | −−− | −−− | −−− |
| Biscuit 8 | −−− | −−− | −−− | Candy 8 | −−− | −−− | −−− |
Note: “+”, positive; “−”, negative. Each cell in the table indicates three results for the presence or absence of peanut, sesame and soybean. For example, “+ − −” shows that the sample contained peanut allergen, but no allergens for sesame and soybean.