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. 2018 Jun 6;8:8671. doi: 10.1038/s41598-018-27003-1

Table 1.

List of sorghum secreted proteins that are responsive to sorbitol-induced osmotic stress.

Prot. #a Accessionb Protein Name Ratioc SDd p valuee Signal Peptidef Family nameg
Glycosyl-hydrolases/Glycosidases
6 A0A1B6QHZ6 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G089000 2.93 0.12 4.85E-06 − Glycoside hydrolase superfamily
8 C5X532 Alpha-galactosidase OS = Sorghum bicolor GN = SORBI_002G123100 2.05 0.05 7.96E-04 + Glycoside hydrolase superfamily
22 A0A1B6QI05 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G089100 2.19 0.08 1.79E-04 + Glycoside hydrolase superfamily
27 C5XKE9 Endoglucanase OS = Sorghum bicolor GN = SORBI_003G015700 2.84 0.35 2.25E-05 − Glycoside hydrolase family 9
28 C5Y397 Alpha-mannosidase OS = Sorghum bicolor GN = SORBI_005G132400 4.24 0.28 1.10E-06 + Glycosyl hydrolase family 38
29 C5X8J4 Xyloglucan endotransglucosylase/hydrolase OS = Sorghum bicolor GN = SORBI_002G302000 3.58 0.24 1.42E-06 + Xyloglucan endotransglucosylase/hydrolase
36 C5XB38 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G055600 2.33 0.16 7.22E-05 + Glycoside hydrolase family 18
72 C5X022 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G525000 2.00 0.11 1.51E-04 + Glycoside hydrolase family 28
82 A0A1B6QC86 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G189100 2.07 0.16 6.77E-04 − Glycoside hydrolase family 81
84 C5XFX7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G247000 2.29 0.20 5.08E-05 + Glycoside hydrolase family 5
86 A0A1B6PTQ9 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_005G204700 2.07 0.48 5.04E-03 + Glycoside hydrolase family 28
88 C5XB39 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G055700 2.35 0.11 1.15E-05 + Glycoside hydrolase family 18
95 C5X5L7 Alpha-galactosidase OS = Sorghum bicolor GN = SORBI_002G417800 4.06 0.21 1.34E-06 + Glycoside hydrolase family 27
133 C5WP48 Alpha-mannosidase OS = Sorghum bicolor GN = SORBI_001G268700 3.05 0.31 3.92E-04 + Glycoside hydrolase family 38
140 C5YCY4 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G160700 2.42 0.42 2.17E-04 − Glycosyl hydrolase family 32
141 A0A1B6Q8G8 Uncharacterized protein (Fragment) OS = Sorghum bicolor GN = SORBI_003G440900 2.92 0.11 4.18E-05 − Glycosyl hydrolase family 32
145 C5YBF1 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G132700 2.79 0.29 2.59E-05 + Glycoside hydrolase family 19
150 C5X3W3 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G246400 2.30 0.42 1.16E-03 + Glycoside hydrolase, family 28
Cell wall modifying enzymes
2 C5WSF9 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G301500 3.18 0.19 3.58E-06 + Expansin/Lol pI
17 C5WSF0 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G300800 3.23 0.41 3.53E-05 + Expansin/Lol pI family
33 C5Z0P5 Uncharacterized protein OS = Sorghum bicolor
GN = SORBI_009G055900		 2.95 0,23 3.77E-06 − Fasciclin-like arabinogalactan protein
59 C5WSE5 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G300400 3.14 0.23 7.20E-06 + Expansin/Lol pI
87 C5YVJ7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_009G232100 2.36 0.11 1.38E-06 + Fasciclin 1 domain
Proteases
14 A0A1B6PLA9 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G104300 2.25 0.09 2.28E-05 + Gamma-glutamyl-transpeptidase
20 A0A1B6QMT3 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G348900 3.09 0.24 6.02E-06 + Peptidase S10, serine carboxypeptidase
26 C5XQ74 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G208800 2.05 0.10 4.70E-04 − Aspartic peptidase A1 family
48 A0A1B6PNM7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G242000 3.27 0.13 7.96E-07 + Peptidase C1A
85 C5WT64 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G170700 2.05 0.18 4.15E-04 + Peptidase S8 subtilisin-related
94 C5WXN2 Carboxypeptidase OS = Sorghum bicolor GN = SORBI_001G348800 2.10 0.14 2.50E-04 + Peptidase S10, serine carboxypeptidase
98 C5YNA1 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_007G172100 3.73 0.25 5.00E-06 + Peptidase C1A
122 A0A1B6PHE0 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_007G120800 3.13 0.41 8.07E-05 − Peptidase M1 family
136 C5WQK1 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G280000 2.77 0.28 3.61E-05 + Peptidase S10, serine carboxypeptidase
138 A0A1B6QEG2 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G315800 4.42 0.25 3.79E-07 + Peptidase C1A
173 C5XDR4 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G217200 2.87 0.25 8.52E-06 + Peptidase C1A
178 C5Y171 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_004G142800 5.62 0.52 1.25E-06 + Peptidase C1A domain and family
Redox proteins
7 A0A1B6QG95 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G416600 2.08 0.03 6.75E-05 − Plant peroxidase
13 C5Y360 Peroxidase OS = Sorghum bicolor GN = SORBI_005G011300 2.73 0.31 4.80E-05 + Plant peroxidase
23 C5Z240 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_010G003100 2.40 0.19 3.69E-05 + Cupredoxin
30 C5WNY4 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G129700 2.07 0.18 1.57E-04 + Germin
31 C5YC92 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G018100 2.18 0.30 3.17E-04 + Germin
35 C5XIY1 Peroxidase OS = Sorghum bicolor GN = SORBI_003G152100 2.98 0.14 5.62E-06 + Plant peroxidase
38 A0A1B6QN00 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G360500 2.16 0.41 1.46E-03 + Plant peroxidase
41 C6JSB7 Peroxidase OS = Sorghum bicolor GN = Sb0246s002010 7.79 1.84 4.99E-05 + Plant peroxidase
51 A0A1B6QGB6 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G416800 2.21 0.07 1.86E-05 + Plant peroxidase
69 A0A1B6Q9F4 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G057900 5.63 0.26 6.39E-08 − Thioredoxin
92 C5XL59 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G024700 −2.40 0.04 7.37E-04 − Plant peroxidase
97 C5XIY0 Peroxidase OS = Sorghum bicolor GN = SORBI_003G152000 2.58 0.11 9.88E-06 − Plant peroxidase
104 A0A194YU12 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_004G341200 6.45 0.29 2.34E-08 − Glutathione-disulphide reductase
110 A0A1B6QN96 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G371900 13.59 1.99 1.14E-05 − Cu-Zn superoxide dismutase-like
111 A0A1B6Q818 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G416300 5.84 0.45 3.19E-07 − GST C-terminal domain-like
129 C5X6P7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G140400 2.34 0.14 1.53E-05 + Cupredoxin
131 C5WWQ2 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G342600 8.47 2.43 6.43E-05 − Thioredoxin
134 C5YQ75 Peroxidase OS = Sorghum bicolor GN = SORBI_008G010500 2.85 0.13 2.98E-06 + Plant peroxidase
137 C5X780 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G007200 2.70 0.12 9.70E-06 + Cupredoxin
151 C5XC95 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G345800 2.76 0.18 1.45E-05 + Cupredoxin
155 A0A1B6QFT7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G392300 38.70 5.94 6.01E-06 + Plant peroxidase
159 A0A1B6P9F6 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_009G190800 4.28 0.35 1.95E-06 − Thioredoxin
161 C5Z0N9 Peroxidase OS = Sorghum bicolor GN = SORBI_009G055300 2.76 0.11 9.30E-06 + Plant peroxidase
167 C5XRU7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_004G148100 3.01 0.42 8.74E-05 + Germin
169 A0A1B6QJR7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G189000 2.10 0.39 2.17E-03 − Plant peroxidase
174 C5YN91 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_007G171000 3.42 2.19 8.12E-03 − FAD/NAD linked reductases, dimerization (C-terminal) domain
179 A0A1B6QB11 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G133800 5.62 0.92 1.42E-05 − FAD/NAD linked reductases, dimerization (C-terminal) domain
Unclassified
19 A0A194YMM6 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_010G262500 7.94 0.27 1.04E-08 − Glyceraldehyde-3-phosphate dehydrogenase, type I
21 C5Z6U2 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_010G210000 2.82 0.36 5.01E-05 − Ubiquitin
40 C5XWE5 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_004G197600 2.52 0.16 3.59E-05 + Glycerophosphoryl diester phosphodiesterase family
43 A0A1B6PD28 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_008G113000 3.08 0.18 7.65E-06 + Purple acid phosphatase, N-terminal domain family
47 C5XPK9 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G205600 2.69 0.09 8.53E-07 + Leucine-rich repeat domain family
49 A0A194YGY2 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_010G027000 5.90 0.35 1.21E-07 − Enolase-like
53 C5Z6U1 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_010G209900 2.96 0.16 5.80E-06 + Not predicted
67 C5Y587 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_005G049800 5.06 0.47 1.20E-06 − Alginate lyase
68 C5YBH7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G135500 2.07 0.07 1.38E-04 + Galactose oxidase central domain
70 C5XX52 Glyceraldehyde-3-phosphate dehydrogenase OS = Sorghum bicolor GN = SORBI_004G205100 4.49 0.29 5.19E-07 − Glyceraldehyde 3-phosphate dehydrogenase
76 C5WXD7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G209300 2.67 0.13 2.62E-05 + Uncharacterised protein family, basic secretory protein
79 C5X502 Dirigent protein OS = Sorghum bicolor GN = SORBI_002G119900 3.01 0.31 2.02E-05 + Allene oxide cyclase/Dirigent protein
90 A0A1B6QEI0 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G317600 4.45 0.12 9.13E-08 − YjgF/YER057c/UK114 family
103 C5YW21 Malate dehydrogenase OS = Sorghum bicolor GN = SORBI_009G240700 5.43 0.89 2.77E-05 − L-Lactate/malate dehydrogenase
106 C5WT90 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G173300 2.48 0.28 2.43E-04 − Reversibly glycosylated polypeptide family
113 C5XYB4 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_004G229300 2.28 0.14 3.92E-05 − Phosphate-induced protein 1
115 C5XQW7 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G087300 2.29 0.12 8.67E-05 + S1/P1 nuclease family
116 C5WQH5 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G149500 2.79 0.48 2.33E-04 − None predicted
117 C5Y1P6 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_005G099500 2.44 0.15 3.59E-05 + Nucleoside phosphatase GDA1/CD39 family
120 C5YSB1 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_008G048400 2.55 0.21 2.60E-05 + Alginate lyase
121 A0A1B6QAK5 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G113800 2.97 0.29 3.91E-05 − Spermidine/spermine synthases
123 C5XFH6 Fructose-bisphosphate aldolase
OS = Sorghum bicolor GN = SORBI_003G393900		 4.01 0.53 2.75E-05 − Fructose-bisphosphate aldolase, class-I
130 C5XTG0 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_004G166500 6.10 0.45 5.54E-07 − N-carbamoylputrescine amidase
132 C5X9N2 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_002G039000 4.15 0.38 4.03E-06 + ML domain
139 C5YRS3 Purple acid phosphatase OS = Sorghum bicolor GN = SORBI_008G037000 3.69 0.34 5.42E-06 − Purple acid phosphatase-like, N-terminal domain family
148 C5WT45 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_001G168500 3.55 0.33 6.16E-06 − Serpin family
152 C5XQ07 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_003G072300 5.32 1.29 1.52E-04 − Triosephosphate isomerase
165 A0A1B6PLT5 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G133000 2.14 0.38 5.89E-04 + Galactose-binding domain-like
168 C5XG88 Small ubiquitin-related modifier 6.26 1.94 6.02E-04 − Ubiquitin-related
181 A0A1B6PJF1 Uncharacterized protein OS = Sorghum bicolor GN = SORBI_006G014400 2.20 0.32 8.41E-04 − AmbAllergen

aProtein number assigned in ProteinPilot.

bProtein accession numbers obtained from the UniProt database searches against sequences of S. bicolor only.

cRatio represents the average fold-change (n = 4) in response to sorbitol-induced osmotic stress relative to the control. A negative value indicates down-regulation.

dStandard deviation of the fold-changes (n = 4).

eProbability value obtained from a Student’s t-test comparing the fold changes between the sorbitol-induced osmotic stress treatments and the control (n = 4).

fSignal peptide prediction using SignalP 4.1 (http://www.cbs.dtu.dk/services/SignalP). A positive sign denotes the presence of a predicted signal peptide; a negative sign denotes the absence of a signal peptide.

gFamily name as predicted using the InterPro (http://www.ebi.ac.uk/interpro/) and Superfamily (www.supfam.org) database.