Fig. 1.

Physical interaction of group A PP2Cs with PYR1. a Yeast two-hybrid analysis of group A PP2Cs with PYR1. Y2H gold cells transformed with GAL4BD-PYR1 and GAL4AD-PP2Cs, as indicated. A series of tenfold serial dilutions were spotted onto DDO (Double dropout medium: SD/-Leu/-Trp), QDO/A (Quadruple dropout medium: SD/-Ade/-His/-Leu/-Trp supplemented with Aureobasidin A) and QDO/A/ABA (Quadruple dropout medium: SD/-Ade/-His/-Leu/-Trp supplemented with Aureobasidin A and ABA) medium agar plates for 7 days after inoculation. b Interaction of PYR1 with AHG1 and AHG3. HA-PYR1 co-immunoprecipitates with YFP-AHG3, but not YFP-AHG1, in an ABA-dependent manner. Total protein extracts from transformed N. benthamiana leaves were harvested 4 days after inoculation and were treated with or without 100 μM ABA for 24 h before harvesting. After co-immunoprecipitation using anti-HA matrix, the input and the immunoprecipitated samples were detected with anti-GFP and anti-HA antibodies