Fig. 4.

CxGel adhesion to cell culture dishes via the adhesion of cells at the surface. (left panels) Photographs of CxGels, prepared through click reaction between azide-modified C2C12 cells (2.0 × 10⁶ cells) and bAlg-DBCO solution (2%), adhered to collagen-coated dishes via the adhesion of cells in the CxGels. After trypsin-EDTA treatment, the adhering cells detached from the dish and the CxGels detached. The CxGels adhered onto a collagen-coated dish again when placed on the dish and cultured in DMEM for 24 h. (right panel) In contrast, since cells in the CxGels, prepared through click reaction between azide-modified C2C12 cells (2.0 × 10⁶ cells) and bAlg-DBCO solution (2%), did not adhere onto MPC polymer-coated dishes, the CxGel also did not adhere onto MPC polymer-coated dishes even after 24 h. Scale bars indicate 20 μm