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. 2018 Jun 6;8:8681. doi: 10.1038/s41598-018-26813-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitation of extravasation of sodium fluorescein for 15 minutes (t0), 4 (t4), and 24 (t24) hours post- 180 kPa blast exposure in frontal cortex, striatum, somatosensory barrel-field cortex, hippocampus, and thalamus using a semilog plot in order to capture magnitudinal differences (A). The frontal cortex was chosen for illustrative purposes and to qualitatively depict the difference between 4 (B) and 24 hrs (C). Arrows indicate areas of leakage from the vessels, which are more pronounced in 4 hours than any other time point studied in this investigation. **indicates a difference in intensity compared with control with a statistical significance of p < 0.01. Scale bar = 100 μm.