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. 2018 Jun 6;8:8689. doi: 10.1038/s41598-018-26826-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Presence of CB1R, NAPE-PLD, or FAAH in the NAc of three vervet monkeys. WB analysis of total protein samples for the CB1R antibody (a) showing detection of the expected major protein band at 60 kDa. For the NAPE-PLD antibody (b), the expected band is seen at 46 kDa, and not detected when pre-incubated with its blocking peptide. For the FAAH antibody (c), the expected band is seen at 63 kDa, and not detected when pre-incubated with its blocking peptide. All lanes contained 10 µg of total protein. The lower blots show the expression of GAPDH and demonstrate loading in all lanes.