Table 1.
Antibody | Immunogen | Source | Working Dilution | RRID |
---|---|---|---|---|
CB | Recombinant protein specific to amino terminus of human CB | Cell Signaling Technology, Danvers, MA, USA | DAB 1:500, IF 1:200 | AB_2687400 |
CB1R | Fusion protein containing aa 1–77 of rat CB1R | Calbiochem, Gibbstown, NJ, USA | DAB 1:300, IF 1:200, WB 1:1,000 | AB_211563 |
CTIP2 | Synthetic peptide corresponding to aa 1–150 of human Ctip2 | Abcam plc., Cambridge, UK | IF 1:200 | AB_2064130 |
FAAH | Synthetic peptide corresponding to aa 561–579 of rat FAAH | Cayman Chemical, Ann Arbor, MI, USA | DAB 1:200, IF 1:200, WB 1:1,000 | AB_10078701 |
GFAP | GFAP purified from pig spinal cord | Cell Signaling Technology, Danvers, MA, USA | IF 1:200 | AB_561049 |
NAPE-PLD | Synthetic peptide from human NAPE-PLD aa 159–172 | Cayman Chemical, Ann Arbor, MI, USA | DAB 1:200, IF 1:200, WB 1:1,000 | AB_10507996 |
PV | Parvalbumin purified from carp muscle | Swant, Marly, Fribourg, Switzerland | IF 1:200 | AB_10000343 |
TH | TH purified from PC12 cells derived from rat pheochromocytoma; recognizes an epitope on the outside of the regulatory N-terminus of TH | EMD Millipore, Chemicon, Temecula, CA, USA | IF 1:200 | AB_2201528 |
CB: Calbindin-d28k; CB1R: cannabinoid receptor type 1; CTIP2: CTIP2 transcription factor; DAB: 3,3′-diaminobenzidine immunostaining; FAAH: fatty acid amide hydrolase; GFAP: glial fibrillary acidic protein; IF: immunofluorescence; NAPE-PLD: N-acyl phosphatidylethanolamine-specific phospholipase D; PV: parvalbumin; TH: tyrosine hydroxylase; WB: Western blot.