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. 2018 May 30;9:718. doi: 10.3389/fpls.2018.00718

FIGURE 2.

FIGURE 2

Phenotype tests of wild type, atunc-93 and AtUNC-93-overexpressing seedlings under salt stress. (A) Schematic structure of AtUNC-93 and the positions of the T-DNA insertion of atunc-93-1 and atunc-93-2. The boxes indicate exons, and the lines represent introns and untranslated regions. (B) Genomic identification of atunc-93-1 and atunc-93-2. LP, left primer; RP, right primer; LB1 and LBb1.3, the T-DNA left border primer. WT is the wild type. (C) Reverse transcription (RT)-PCR verification of AtUNC-93 expression in atunc-93 mutants. (D,E) Phenotype (D) and the survival rates (E) of atunc-93 mutant seedlings and the wild type grown on 1/2 MS medium containing 100, 125, and 150 mM NaCl for 14 days, respectively. (F) Real-time PCR verification of AtUNC-93 in 35S:AtUNC-93 transgenic lines (OE1, OE2, and OE3). (G,H) Phenotype (G) and the survival rates (H) of AtUNC-93-overexpressing lines and the wild type grown on 1/2 MS medium containing 100 and 125 mM NaCl for 14 days, respectively. Data in (E,F,H) represent the means ± SE (n = 3). The student’s t-test (∗∗P < 0.01) was used to analyze statistical significance compared with the wild type.