Table 5.

Seven eSSR primers optimized and used to assess population genetics in kudzu accessions

Locus	Sequence	Dye/Tail	SSR	Length (bp)
PP2	F: 5′-TAG GAG TGC AGC AAG CAT ATG CCG CGG ATC TTT GAA AG-3’	VIC /M13A	AAC	100–130
	R: 5’-CAA ATT GGC CCT GTC CCA AT-3’	n/a
PP4	F: 5′-TGT AAA ACG ACG GCC AGT CAT GCC CAC GTG CTT CAT AG-3’	6FAM/M13	GCT	100–140
	R: 5’-CTC TCA GAT CCA GGC CCA AA-3’	n/a
PP10	F: 5′-TAG GAG TGC AGC AAG CAT GGC ATG TAG ATC CAG CTA AA-3’	VIC/M13A	GGT	310–330
	R: 5′-TTG ACA GAT TTC TGA TTC TTG G-3’	n/a
PP13	F: 5′-TAG GAG TGC AGC AAG CAT GAT TGA GCA GGC ACG AGA AC-3’	VIC/M13A	GCT	270–300
	R: 5’-CAG TAG CAG GCA TGT GTT GG-3’	n/a
PL1	F: 5’-CAC TGC TTA GAG CGA TGC TGT AAG CGT TCG TTC GTT GG-3’	PET/M13B	CTT	400–440
	R: 5’-TCA ACC TGG TGC TCT CTG AC-3’	n/a
PL7	F: 5′-TGT AAA ACG ACG GCC AGT AGT GGC CTT GCT CTT CTT CC-3’	6FAM/M13	CTT	80–140
	R: 5′-GTG TCA TCT CAG CAC GTT GG-3’	n/a
PL11	F: 5′-TGT AAA ACG ACG GCC AGT TGG CAT CAT CCT TCA ACC AC-3’	6FAM/M13	ACC	300–330
	R: 5′-ATT CGG GAA TAG TGG GTG GG-3’	n/a

F forward primer, R reverse primer. Dyes VIC: 2′-chloro-7′phenyl-1,4-dichloro-6-carboxy-fluorescein; 6FAM: 6-carboxyfluorescein; PET: chemical structure currently unpublished as proporietary to Lifetech. Tail: see Culley et al. [19] for information about M13, M13A, and M13B