Table 2.

Sequences of forward and reverse primers for amplification of apple genes used in the Bisulfite sequencing-PCR analyses

Primer position	Sense Primer (5′-3′)	Anti-sense Primer (5′-3′)
−2026, − 1870a	GAAATYGTTYGAAGGTYTAAGG	ACARCAAACACCCAAAATCC
−1898, −1633	TGATTAAAGGGATTTTGGGTGTTTG	AAATARAACCRRACCTATCTCA
−1667, −1287	GTTTGTAAYAGAYTGAGATAGGT	TCCAAAACCCCARATTCTTAT
−1312, −1035	TATGYATAAGAATYTGGGGTTTTGGA	CTTACTTRRTCACATTATACCATC
−1062, −964	YAYYGATGGTATAATGTGAYYAAGT	ACATTATTTTCATARRTRRTTCARCC
−989, −774a	GGYTGAAYYAYYTATGAAAATAATG	ARACRCTACACCTAACACATTRCT
−841, −533	TTGTGAAAGYTTAGTGAGTTGAAG	ATTCTCTCCTTTTTTTCCCTCTTCT
−541,-435	GGAGAGAATYYTAYTYYATAAATTAYAAG	CTTTCRCTRCTTTTTCAARTRTT
−434, −188	TTATGGTGGTYAAAGATGTGTGTTGTAA	CAAACACTRACAARTTTAAAACATTCCAACA
−198, −60	GTYAGTGTTTGYTTYTGTGGATATY	TCCARAAARACACCTARCTACC
−51, 106a	AGTGGGTAGYAGGYAAAAGA	TCCACTTTCCCTCTCCATRA

^aPrimers modified from Telias et al. [11]. Y = C or T; and R = A or G