Table 1.
Protein | Dilution | Incubation time | Incubation Temperature | Company | Location |
---|---|---|---|---|---|
CGRP | 1:1000 | 3 Hours | 20–22°C | Sigma-Aldrich, Inc. | St. Louis, MO |
Iba-1 | 1:400 | 3 Hours | 20–22°C | Wako Chemicals USA, Inc. | Richmond, VA |
NeuN | 1:1000 | 3 Hours | 20–22°C | EMD Millipore, Corp. | Temecula, CA |
p-ERK | 1:500 | OVN | 4°C | Bioworld Technology, Inc. | St. Louis Park, MN |
Active PKA | 1:500 | 3 Hours | 20–22°C | Abcam, Inc. | Cambridge, MA |
Alexa-Fluor 488 | 1:200 | 1 Hour | 20–22°C | Jackson Immuno Research, Inc. | West Grove, PA |
Alexa-Fluor 568 | 1:200 | 1 Hour | 20–22°C | Life Technologies | Grand Island, NY |
Alexa-Fluor 647 | 1:200 | 1 Hour | 20–22°C | Life Technologies | Grand Island, NY |