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. 2018 May 19;18:e00258. doi: 10.1016/j.btre.2018.e00258

Table 2.

Km and Vmax values for free and immobilized systems.

Enzyme type Km (mM) Vmax μmol (min mg protein)−1
Free enzyme 12.5 33,000
Catalase immobilized on chitosan beads 25 26,300
Catalase immobilized on chitosan-bentonite complex beads 20 24,500