Table 4.
PCR analysis of repair products
| Strain | Number of colonies analyzed | Approximate deletion sizes in bp in Luc- colonies | |||
|---|---|---|---|---|---|
| ≤50 | 51–500 | 501–1000 | >1000 | ||
| LHmKumLig | 67 | 5 (7.5%) | 9 (13.4%) | 10 (14.9%) | 43 (64.2%) |
| LHtKumLig* | 83 | 23 (27.7%) | 23 (27.7%) | 9 (10.8%) | 28 (33.7%) |
| LHmKutLig* | 50 | 13 (26%) | 6 (12%) | 8 (16%) | 23 (46%) |
Cla I-linearized DNA was transformed into the indicated strains and the CarbR colonies were tested for luciferase activity. The deletions in pBestluc in the Luc- colonies were determined using PCR followed by electrophoresis of PCR products through an agarose gel.
*P < 0.05 compared to LHmKumLig.