Table 5.

Sequence of repair products from LHmKumLig

Deletion size	Sequence before repair	Sequence after repair	Microhomology region
12 bp	GAATTG−ATTGTTA	GAATTGTTA	ATTG
60 bp	CCGCTTG−GTTACAA	CCGCTTGTTACAA	G
134 bp	GTTGACCGC−CCGCCGCC	GTTGACCGCCGCC	CCGC
661 bp	GGTTGCAAA−CAAATTG	GGTTGCAAATTG	CAAA

LHmKumLig bacteria were transformed with Cla I-linearized DNA and the Luc^- Carb^R colonies were tested for deletions in pBestluc. Colonies were selected that contained a range in size of deletions and the pBestLuc was isolated from these bacteria and sequenced. The junctions of the deletion were identified and are shown above. The dash (−) in the ‘sequence before repair’ relates to the sequence between the microhomology regions in pBestluc. The microhomology regions are shown in bold.