Figure 2.
FGF2 Is Permissive for Rarg Expression in Cultured Spermatogonial Cell Lines
(A) Schematic representation of the phenotype of spermatogonial subsets and SSC cell lines based on previous reports (Gely-Pernot et al., 2012, Hara et al., 2014, Ikami et al., 2015, Takashima et al., 2015, Garbuzov et al., 2018).
(B and C) Effects of FGF2 and GDNF on Rarg expression in GS cells. (B) GS cell culture conditions. Cells were cultured on laminin-coated dishes. (C) qRT-PCR analysis. After normalization to Hprt expression, values of FGF2 + GDNF were set to 1.0 (n = 8 independent cultures for each group).
(D) qRT-PCR analysis of Rarg expression in cultured spermatogonial cell lines. After normalization to Hprt expression, the value of GS cells was set to 1.0 (GS cells, n = 13 independent cultures; F-SPG, n = 14 independent cultures; G-SPG, n = 18 independent cultures). Results were obtained from three (GS cells and F-SPG) and four (G-SPG) independently established sublines for each group.
Results are presented as means ± SEM. Daggers indicate statistical significance (†p < 0.05).