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. 2018 Apr 26;10(6):1851–1866. doi: 10.1016/j.stemcr.2018.03.023

Figure 2.

Figure 2

Analysis of hPSC Cell Cycle and Cyclins during CHIR Induction

(A) Cell-cycle profile of HES3 cultures after induction with 0–12 μM CHIR, and cell-cycle profile of hPSCs cultured at 50%, 70%, and 90% culture confluency after 12 μM CHIR induction (n = 3).

(B) Nuclei; DAPI (blue) and spindle apparatus of mitotic cells; γ-tubulin (red) staining of monolayer HES3 cultures treated for 8 hr with 0–12 μM CHIR. Numbers in brackets give the percentage of positively stained cells (n = 3 with 10 regions of interest each, ∗p ≤ 0.05).

(C) Whole-cell protein blot expressions and quantitative analyses of HES3 cells after 4–12 μM CHIR induction.

(D) Whole-cell protein blot expressions and quantitative analyses of HES3 cultures at 50%, 70%, and 90% culture confluency after 12 μM CHIR induction.

(E) Cell number of hPSC cultures at 50%, 70%, and 90% culture confluency after 12 μM CHIR treatment (n = 3, ∗p ≤ 0.05).