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. 2018 Apr 26;10(6):1851–1866. doi: 10.1016/j.stemcr.2018.03.023

Figure 3.

Figure 3

Analysis of hPSC Cell Cycles and Pluripotency in Relationship to Cardiac Differentiation with CHIR

(A and B) Day 11 single-EB GFP/NKX2-5 expression area and percentage of differentiated (induction for 24 hr with 6 μM CHIR) HES3 cells cultured at <50%, 50%–70%, 70%–90%, and >90% culture confluency of low to high S/G2/M cell-cycle profiles (n = 8) (A). Day 11 Troponin T flow cytometry of EBs from differentiated IMR90 cell cultures with a 7% difference in S/G2/M cell-cycle profile (n = 4, ∗p ≤ 0.05) (B).

(C) Cell-cycle profiles of HES3 cells and G1/S cell-cycle-arrested cells. Troponin T expression of differentiated HES3 with CHIR on day 14 (n = 3, ∗p ≤ 0.05).

(D) Average pluripotency flow cytometer population of pluripotency markers and the percentage of positive population of the S/G2/M cell-cycle profile in hPSCs (n = 12).

(E) Average pluripotency flow cytometer population of pluripotency markers and the percentage of positive population of the S/G2/M cell-cycle profile in hPSCs (n = 3, ∗p ≤ 0.05).

(F) Asymptotic correlation of NANOG and OCT4a flow cytometry population (%) of 17 monolayer hPSC cell cultures on day 0 against peak Troponin T cardiomyocyte population on day 14 after 4–12 μM CHIR induction.

(G) Correlation of peak Troponin T cardiomyocyte population on day 14 after 4–12 μM CHIR induction of 17 independent monolayer hPSC cultures against S cell-cycle phase (%) on day 0.