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. 2018 Apr 26;10(6):1851–1866. doi: 10.1016/j.stemcr.2018.03.023

Figure 4.

Figure 4

β-Catenin and TCF Modulations after CHIR Induction

(A) Whole-cell protein blot expressions and quantitative analyses of HES3 cells cultured at <50%, 50%–70%, 70%–90%, and >90% cell culture confluency after CHIR induction.

(B) Fluorescence microscopy of immuno-cytochemistry-stained HES3 cells against active β-catenin (green) and DAPI (blue) after CHIR induction.

(C) Nuclear fraction protein blot expressions and quantitative analyses of HES3 EBs after CHIR induction. Note sample CHIR 0 μM degraded after 24 hr.

(D) Whole-cell protein blot expressions and quantitative analyses of HES3 EBs after CHIR induction.

(E) Normalized TCF7L1/2 protein expression folds of hPSCs cultured at 90% cell confluency with increased G1 cell-cycle phase compared with 70% cell confluency, with an increased S/G2/M and average TCF7L1/2 protein expressions of hPSCs after G1/S cell-cycle arrest (n = 3, ∗p ≤ 0.05).