Figure 5.
Acceleration of Mesodermal Development after CHIR Induction
(A) qPCR analyses of fold increase/decrease over HES3 control after treatment of HES3 EBs with 3 μM CHIR, DMSO control, and 5 μM Wnt inhibitor TA-01 for 24 hr (n = 3).
(B) Whole-cell protein blot expressions and quantitative analyses of HES3 cells and differentiating EBs after CHIR induction.