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. 2018 Apr 26;10(6):1851–1866. doi: 10.1016/j.stemcr.2018.03.023

Figure 6.

Figure 6

TCF7L1/2 Expression Regulates Wnt Inhibition and Early Cardiac Differentiation

(A and B) Induction timing for CHIR and Wnt inhibitors during the differentiation process (A). Differentiated HES3 EBs on day 11 expressing GF/NKX2-5 area in white and GFP/NKX2-5 population percentage (B). HES3 EBs were cultured with 6 μM CHIR for 24 hr and with/without 2.5 μM IWR-1 induction on day 1–3 (n = 4, ∗p ≤ 0.05).

(C) Whole-cell protein blot expressions and quantitative analyses of HES3 EBs after 6 μM CHIR induction (ctl.) and 2.5 μM IWR-1 inhibition at day 1 (inh. D1) and day 2 (inh. D2).

(D) Protein blot expressions and quantitative analyses of whole-cell HES3 EBs after 6 μM CHIR induction (ctl.) and 2.5 μM IWR-1 inhibition at day 1 (inh. D1) and day 2 (inh. D2).