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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Cancer Cell. 2012 Sep 11;22(3):373–388. doi: 10.1016/j.ccr.2012.07.016

Figure 6. Notch and Hedgehog Signaling Regulate Survival Molecules in Docetaxel-Resistant Cells.

Figure 6

(A) Immunoblots of indicated proteins in CK19/GFP− and CK19/GFP+ sorted cells from DU145-pCK19-GFP and 22Rv1-pCK19-GFP.

(B) Immunoblots of indicated proteins in DU145-pCK19-GFP− and 22Rv1-pCK19-GFP− cells exposed for 72 hr to DBZ and/or Cyclopamine (both 1 μM).

(C) Immunoblots of indicated proteins in DU145-pCK19-GFP− and 22Rv1-pCK19-GFP− cells exposed for 72 hr to LY294002 (50 μM) and/or ABT-737 (10 μM).

(D) Colony formation assay and quantification of colonies derived from GFP− and GFP+ cells exposed to the same drugs and concentrations as in (C).

(E) Flow cytometry analysis after 48 hr administration of Cyclopamine (1 μM) and/or DBZ (1 μM) of DU145-pCK19-GFP and 22Rv1-pCK19-GFP cells stably transfected with empty vector (EV), MYR-AKT and BCL2. Immunoblots illustrate the overexpression levels of pAKT (Ser-473) and Bcl-2.

(F) Colony formation assay and quantification of DU145-pCK19-GFP- and 22Rv1-pCK19-GFP-sorted cells stably transfected with empty vector (EV), MYR-AKT, or BCL2 and exposed for 72 hr to Cyclopamine (Cyc), GDC-0449 (GDC), DBZ, and Compound-E (CE, all 1 μM), alone or in combination.

(G) Colony formation assays and quantifications of GFP+-sorted DU145-pCK19-GFP and 22Rv1-pCK19-GFP stably transfected with EV, MYR-AKT, or BCL2 and treated for 72 hr with Mitoxantrone 125 and 500 nM, Cisplatin 5 and 2.5 μM, and Vinorelbine 500 and 750 nM, respectively. Data is represented as means ± SD of three independent experiments. *p < 0.05.

See also Figure S6.