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. 2018 May 22;109(6):1753–1763. doi: 10.1111/cas.13618

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© 2018 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Aggressive characteristics of 4T1, 4T1/NC and 4T1/sPD‐1 cells. A,B, 5‐Ethynyl‐2′‐deoxyuridine (EdU) assay was carried out to assess proliferation ability among 4T1, 4T1/NC and 4T1/sPD‐1 cells. NC, negative control; sPD‐1, soluble programmed death receptor‐1. C,D, Transwell assay was conducted to compare invasion ability among 4T1, 4T1/NC and 4T1/sPD‐1 cells at 12 h. E,F, Wound healing assay was used to assess migration ability among 4T1, 4T1/NC and 4T1/sPD‐1 cells at 12 and 24 h (magnification ×200) (ns, P > .05)