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. 2018 Apr 16;19(6):e45176. doi: 10.15252/embr.201745176

Figure 2. LGP2 inhibits signaling downstream of MAVS.

Figure 2

  1. IFNβ‐reporter gene assay with 6 h poly(I:C) (5 μg/ml) stimulation with or without LGP2 expression. 2fTGH cells were harvested for luciferase assays.
  2. Similar to (A), but using PRDII (NFκB) reporter gene.
  3. Similar to (A), but using PRDIII/I (IRF3) reporter gene.
  4. IFNβ‐reporter gene assay with MAVS expressed with or without LGP2 titration. Cells were harvested 24 h post‐transfection for luciferase assays.
  5. Similar to (D), but using PRDII (NFκB) reporter gene.
  6. Similar to (D), but using PRDIII/I (IRF3) reporter gene.
  7. ISRE‐reporter gene assay with 6‐h IFNα stimulation with or without LGP2 titration.
  8. GAS‐reporter gene assay with 6‐h IFNγ stimulation with or without LGP2 titration.
Data information: Bars represent average values (n = 3) ± standard deviation. Corresponding immunoblots in Fig EV1. ***P ≤ 0.0005, **P ≤ 0.005, and *P ≤ 0.05 by two‐tailed Student's t‐test.