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. 2018 Apr 16;19(6):e45176. doi: 10.15252/embr.201745176

Figure 5. LGP2 inhibits MAVS‐independent TRAF‐mediated signaling.

Figure 5

  1. NFκB‐reporter gene assay with HEK293 cells treated (+) for 6 h with TNFα (10 ng/ml) with or without LGP2 plasmid titration as indicated. Bars represent average values (n = 3) ± standard deviation. Corresponding immunoblots in Fig EV1.
  2. HeLa cells transfected with indicated amounts of LGP2 or empty vector were treated with TNFα (10 ng/ml) for 5 min. Cell lysates were subjected to immunoblotting with antibodies that recognize phospho‐IκBα (ser32), total IκBα, FLAG‐tagged LGP2, and GAPDH.
  3. Similar to (A), but treating HEK293 cells with IL‐1β (10 ng/ml)
  4. Similar to (A), but STING was co‐transfected with indicated amounts of the LGP2 plasmid prior to stimulation with 2′3′ cGAMP (20 μg/ml) for 24 h in HEK293 cells.
Data information: ***P ≤ 0.0005 and **P ≤ 0.005 by two‐tailed Student's t‐test.Source data are available online for this figure.