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. 2018 Jan 29;9(3):557–569. doi: 10.1002/jcsm.12283

Figure 4.

Figure 4

Influence of G93A hmSOD1 gene mutation on cellular level of ferritin L and H and iron in SH‐SY5Y cell line. Representative immunoblot for (A) ferritin L, (B) ferritin H, and (C) SOD1 in stable cell line SH‐SY5Y expressing pcDNA3.1, G93A SOD1, or wild‐type (WT) SOD1. Changes in ferritin L and H densitometry levels were normalized against β‐actin. Experiments were performed at least four independent times, yielding comparable results. Bar graphs on the right panel of the figure present a change in protein levels as the mean ± SEM of four independent experiments, relative to control. (D) Non‐haem iron accumulates in the cells expressing SOD1 G93A. Non‐haem iron levels in cells expressing SOD1 G93A, WT SOD1, and pcDNA3.1. Data are presented as mean ± SEM (n = 3), where a, P < 0.05 compared with the pcDNA3.1 and b, P < 0.05 compared with WT SOD1.