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An example for membrane protein and intracellular protein visualization with cell permeable and impermeable fluorogens. (a) The chemical structure of the cell excluded MG-BTau (4), and cell permeable MHN-ester (7). (b) Schematized paradigm for GIRO labeling. (c) Live cells labeled with MG-BTau (red) and MHN-Ester (green) to label surface and internal protein in FAP-BKα expressing live cells. Adapted with permission from Ref. 28. Copyright (2015) American Chemical Society.
