Table 1.
Properties of FAPs and fluorogens. Fluorogens are derivatives of MG or TO, FAPs are comprised of hypervariable heavy (H) and light (L) chains joined by a flexible linker. All information is taken from Refs. 18, 21.
| FAP | Fluorogen | Size (kDa) | Excitation λmax (nm) | Emission λmax (nm) | Quantum yield | Fluorescence enhancement |
|---|---|---|---|---|---|---|
| L5-MG | MG-2p | 11.5 | 640 | 668 | 0.048 | 4100 |
| H6-MG | MG-2p | 14.4 | 635 | 656 | 0.25 | 18,000 |
| H8-MG | MG-2p | 13.6 | 626 | 646 | ||
| HL4-MG | MG-2p | 26.1 | 629 | 649 | 0.16 | 15,700 |
| HL1.0.1-TO1 | TO1-2p | 25.9 | 509 | 530 | 0.47 | 2600 |
| L5*-MG | MG-2p | 14.2 | 634 | 667 | 0.24 |