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Photomicrography of 5 μm frozen liver slices containing reperfused liver (left) and muscle slices containing absolute ethanol induced necrosis (right) from the model rats at 12, 24, 72 h and 8 days after intravenous injection of EB (2.0 mg/kg), respectively. Fluorescence emission intensity was recorded at λex=620 nm and λem=680 nm. Corresponding unstained (upper), fluorescence (middle) and H&E stained (under) pictures: N=necrotic area, V=viable area. Scale bar=50 μm.
