Figure 2.

Enhancer of zeste homolog 2 (EZH2) expression is associated with cellular platinum accumulation. A, Cellular platinum (Pt) content of A2780 and ES2 cells with exposure to cisplatin (cDDP) after EZH2 depletion. B,C, Time course accumulation and efflux curve of cDDP in A2780 cells with cDDP exposure after EZH2 depletion. D, Chemical structure of the BODIPY‐Pt complex. E,F, Western blot and quantitative real‐time PCR analyses of EZH2 expression after transfection with EZH2 siRNA (SiEZH2) in A2780 and ES2 cells. G, Representative images of cellular BODIPY‐Pt (green) merged with DAPI (blue) in A2780 and ES2 parent cells and in SiNC (Control) and SiEZH2 cells and the mean intensities of BODIPY‐Pt in each cell line. Bars represent mean ± SD (n = 3). *P < .05, **P < .01, ****P < .0001, Student's t‐test. ShEZH2, EZH2 shRNA; ShNC, scrambled control