Figure 4.

Messenger RNA expression of target genes of miR‐148a‐5p and miR‐148a‐3p on clinical sample and directly regulation of mRNA expression by miR‐148a‐5p and miR‐148a‐3p transfectants in pancreatic ductal adenocarcinoma (PDAC) cell lines. A, Expression levels of miR‐148a‐5p target genes (PHLDA2, LPCAT2, AP1S3) in PDAC clinical specimens and cell lines were determined by qRT‐PCR. GUSB was used as an internal control, data were normalized to GUSB expression (left), *P < .05. Gene expression was directly downregulated by transfection of miR‐148a‐5p mimic (right), *P < .0001. B, Expression levels of miR‐148a‐3p target genes (SMS,ENDOD1,UHMK1) in PDAC clinical specimens and cell lines were determined by qRT‐PCR. GUSB was used as an internal control, data were normalized to GUSB expression (left), *P < .05. Gene expression was directly downregulated by transfection of miR‐148a‐3p mimic (right), *P < .0001