Figure 1.

Plakoglobin (PG) interacts with the mutant p53^R175H and decreases β‐catenin levels. A, Total cell extracts from untransfected H1299 cells and H1299 cells transfected with p53, p53^R175H, PG, PG and p53 (PG‐p53) or PG and p53^R175H (PG‐p53^R175H) were processed for immunoblot using plakoglobin and p53 antibodies. β‐Actin levels in the same extracts were used as an internal control. B, Total cell extracts (TCE) from H1299 cells coexpressing plakoglobin and p53^R175H were processed for reciprocal and sequential coimmunoprecipitation (Co‐IP) and immunoblotting (IB) using p53 and plakoglobin antibodies. C, Total cell extracts from parental H1299 cells and H1299 transfectants expressing p53 or p53^R175H with or without plakoglobin were processed for immunoblot using plakoglobin and β‐catenin antibodies. β‐Actin levels were used as loading control. Histograms represent the average ± SD of 4 separate experiments. All values are normalized to H1299 cells. UT, untransfected. *P < .05 **P < .001