Table 1.
Antibodies and their respective dilutions in specific assays
| Species | Assay | Source/Catalog number | |||
|---|---|---|---|---|---|
| WB | IP | IF | |||
| Primary antibodies | |||||
| p53‐ DO‐1 | Mouse | 1:1000 | 1:100 | ‐ | Santa Cruz Biotechnology/sc‐126 |
| Plakoglobin (γ‐catenin) | Mouse | 1:1000 | 1:100 | 1:100 | BD Transduction Laboratories/610254 |
| β‐catenin | Mouse | 1:1000 | 1:100 | 1:100 | Sigma Aldrich/C‐7207 |
| β‐catenin (nuclear) | Mouse | 1:1000 | ‐ | ‐ | Abcam/ab 19451‐50 |
| TCF‐4 | Mouse | 1:500 | 1:100 | ‐ | Upstate Biotechnology/05‐511 |
| β‐actin | Mouse | 1:1000 | ‐ | ‐ | Santa Cruz Biotechnology/sc‐47778 |
| Lamin B1 | Rabbit | 1:1000 | ‐ | ‐ | Abcam/ab 16048 |
| Secondary antibodies | |||||
| Anti‐mouse, light chain IgG | Goat | 1:20 000 | ‐ | ‐ | Jackson ImmunoResearch/115‐625‐174 |
| Anti‐rabbit, light chain IgG | Goat | 1:20 000 | ‐ | ‐ | Jackson ImmunoResearch/211‐652‐171 |
| Alexa Fluor 488 | Mouse | ‐ | ‐ | 1:2000 | Molecular Probes Biotechnology/A11029 |
| Alexa Fluor 546 | Rabbit | ‐ | ‐ | 1:3000 | Molecular Probes Biotechnology/A11035 |
IF, Immunofluorescence; IP, Immunoprecipitation; WB, western blot; ‐, Not applicable.