Figure 3.
PAI‐1 promoted Src inhibitor resistance, cell proliferation and migration in breast cells. A, Relative cell viability was determined in SKBR‐3 and SKBR‐3/SI cells following treatment with 10 ng/mL rPAI‐1 and PAI‐039 for 72 h by CCK8 assay. IC50 values of SKBR‐3 and SKBR‐3/SI cells were presented in the mini‐tables. B, OD value in SKBR‐3 and SKBR‐3/SI after treatment of 1, 10 or 100 μmol/L rPAI‐1 and PAI‐039, respectively, for 24, 48 and 72 h. C, Colony formation showed the proliferation of SKRB‐3 and SKBR‐3/SI after treatment of 10 ng/mL rPAI‐1 and PAI‐039, respectively. D, Cell apoptosis of SKBR‐3 and SKBR‐3/SI after exposure to 10 ng/mL rPAI‐1 or PAI‐039 for 48 h was shown. E, Cell migration analysis in SKBR‐3 and SKBR‐3/SI cells treated with 10 ng/mL rPAI‐1 and PAI‐039 were performed. The error bars represent the mean of 3 separate determinations ± SD. *P < .05, **P < .01, ***P < .001