Figure 4.

PAI‐1 upregulated the expression of CCL5 in SKBR‐3/SI cells. A, qRT‐PCR verified the increased expression of CCL5 in SKBR‐3/SI cells. B,C, The expression level of CCL5 and the secretion level of CCL5 were detected in SKBR‐3 after treated with 10 ng/mL of rPAI‐1and PAI‐039 for 48 h by qRT‐PCR and ELISA, respectively. D, qRT‐PCR analysis of PAI‐1 mRNA expression post‐siRNA knockdown of PAI‐1 in SKBR‐3/SI cells. E, PAI‐1 and CCL5 expression were determined in SKBR‐3/SI after knockdown of PAI‐1 by western blot. The error bars represent the mean of 3 separate determinations ± SD. *P < .05, **P < .01, ***P < .001