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. 2018 Jan 29;9(3):570–584. doi: 10.1002/jcsm.12288

Figure 4.

Figure 4

IS modulates myoblast and myotube UPR signalling pathways. (A–C) BiP mRNA expression level and phosphorylation of eIF2α are examined in C2C12 myoblasts (MB) after 2 days exposure of IS (1 mM). (D and E) The levels of both XBP1u or XBP1s mRNA were determined by semi‐quantitative PCR. (F–H) BiP mRNA expression level and phosphorylation of eIF2α increased are examined in IS‐treated well myogenic differentiation (MD) cells. (I and J) The level of XBP1u and XBP1s mRNA expression is determined in well MD cells with or without IS (1 mM) for 48 h. The data were expressed as mean ± SED from three independent experiments. *P < 0.05, ** P < 0.01, and *** P < 0.001, as compared with untreated control.