Figure 4.

Insulin secretion from ex vivo fetal pancreas cultures and isolated rat neonatal islets. A) Collagenase-treated fetal pancreas were cultured ex vivo in the absence (white bars) or presence (black bars) of TFF3 (200ng/mL) for 5 days. Each day the culture medium was aspirated and the insulin secreted into the media the preceding 24 hrs was determined. **: p < 0.05 between insulin secretion at Day 1 of culture compared with Days 3 to 5. B) Insulin secretion of neonatal rat islets at 2mM glucose after 1, 25, 49 and 73 hrs of culture with TFF3 (200ng/mL). C) Insulin secretion of neonatal rat islets at 20mM glucose after 1, 25, 49 and 73 hrs of culture with TFF3 (200ng/mL). Data are from 4 experiments performed in replicates of 3.