Figure 3.
Neuroblastoma cell toxicity was present in the purified fraction eluting from protein A following Liposorber/protein-A sequential chromatography, but was lacking in the other fractions (A). The highly purified protein-A eluate fraction of diabetic dementia plasma caused significantly greater dose-dependent suppression of N2A neurite outgrowth compared to the Liposorber eluate fraction from the same patient (B). Dose-dependent N2A cell toxicity in the protein-A eluates from diabetic dementia (Pt 1) and PDD (Pt 3) significantly exceeded toxicity in the corresponding Liposorber DMT eluate fraction (C). ** P < 0.01; * P < 0.05.