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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Am J Transplant. 2009 Apr;9(4):709–718. doi: 10.1111/j.1600-6143.2009.02578.x

Figure 1. Adoptive transfer of TCR-tg T cells allows for simultaneous tracking of three subtypes of alloreactive T cells.

Figure 1

A) TCR-tg T cells representing the CD4-d (4C), CD4-i (TCR75) and CD8-d (2C) pathways of alloantigen recognition were purified by magnetic bead isolation and tested for purity by TCR-Vβ transgene expression and CD4 or CD8 expression using FACS analysis. B) Schematic of T cell purification, labeling with intravital dye (CFSE), and intravenous adoptive transfer into a syngeneic host. C) After transplantation, TCR-tg T cells could be identified amongst host T cells by the congenic cell surface markers, Ly5.1 and Thy1.1.