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. 2018 May 9;7:e36341. doi: 10.7554/eLife.36341

Figure 2. CD8-dependent binding of peptide-deficient HLA-B*35:01 tetramers to CD8+ T cells.

Figure 2.

Uncleaved B*35:01 tetramer poorly stained CD8+ T cells (A and F), whereas peptide-deficient B*35:01 tetramers stained most (over 70%) CD8+ T cells from B*35:01-positive donors (such as Donor 111) (B) or B*35:01-negative donors (such as Donor 133) (G) in a manner sensitive to blockage by anti-CD8 (Clone SK1) (C and H). CD4+ T cells were, in comparison, poorly stained by peptide-deficient B*35:01 tetramers and the staining cannot be blocked by anti-CD8 (D, E, I and J). Peptide-deficient B*35:01 tetramer staining data from 10 tested donors (mean ± SEM of one to two assays) are shown in (K and L).