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. 2018 May 9;7:e36341. doi: 10.7554/eLife.36341

Figure 3. Preferential binding of peptide-deficient conformers of HLA-B*35:01 to CD8.

Figure 3.

(A) Primary NK cells (CD3-CD56+) from Donor 115 were stained with peptide-deficient B*35:01 tetramers, demonstrating specific binding to the CD8+ NK cell fraction (left panel). NK cell staining by peptide-deficient B*35:01 tetramers was blocked by anti-CD8 (right panel). Representative data are shown based on two experiments each with four donors. (B) Primary NK cells from different donors have different CD8+ fractions and CD8-dependent binding of peptide-deficient B*35:01 tetramer to NK cells is proportional to the CD8+ fraction of NK cells among tested donors. The mean ± SEM of two experiments for each donor are shown. (C) Binding of SA-bead immobilized peptide-deficient or peptide-filled B*35:01 to the indicated concentrations of CD8-FITC. Proteins pulled-down were analyzed by SDS-PAGE gel and fluorimaging. (D) Quantified binding signals are plotted following background subtraction. Data are representative of four experiments.