Table 3. Primers for SNP genotyping in PCR and single-base extension reaction of MALDI-TOF MS assay.
Gene(GFAP) Loci |
Chr:position | Forward primera | Reverse primera | Extension primer | Amplicon size(bp) |
rs11558961 | 17:44907319 | linker-TTGCACTGTGCACGTTC | linker- TGGGGAAATGTGCCAG | GGATGTGATGTGA | 165 |
rs1042329 | 17:44906346 | linker-TGCCCCGTGCAGACTGGA | linker- TCCCACAATCCAGAGG | CGTGC AGACTGGA | 119 |
rs8067254 | 17:44907009 | linker- CAGTTCCCAGATAC | linker- GCACCTACTACATC | CTCCATCTCTGGCA | 92 |
rs17027 | 17:44905825 | linker- CTTCTTCGGCCTTAG | linker- GCTTTGACTGAGCAGAC | CCTTAGAGGGGAGA | 106 |
alinker=10-mer linker ACGTTGGATG placed at 5'-end of each primer.