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. 2018 May 10;10(5):988–999. doi: 10.18632/aging.101442

Table 3. Primers for SNP genotyping in PCR and single-base extension reaction of MALDI-TOF MS assay.

Gene(GFAP)
Loci
Chr:position Forward primera Reverse primera Extension primer Amplicon size(bp)
rs11558961 17:44907319 linker-TTGCACTGTGCACGTTC linker- TGGGGAAATGTGCCAG GGATGTGATGTGA 165
rs1042329 17:44906346 linker-TGCCCCGTGCAGACTGGA linker- TCCCACAATCCAGAGG CGTGC AGACTGGA 119
rs8067254 17:44907009 linker- CAGTTCCCAGATAC linker- GCACCTACTACATC CTCCATCTCTGGCA 92
rs17027 17:44905825 linker- CTTCTTCGGCCTTAG linker- GCTTTGACTGAGCAGAC CCTTAGAGGGGAGA 106

alinker=10-mer linker ACGTTGGATG placed at 5'-end of each primer.