. 2018 May 19;10(5):1103–1132. doi: 10.18632/aging.101452

Table 1A. Detailed characteristics of major hallmarks of cellular senescence of the individual donors HDF161, HDF76 and HDF85 each senescent and quiescent control after 7 (D7) days post last H₂O₂ application.

1 week recovery (D7)

Donor

Total apoptotic cells (Annexin + PI positive) [%]

BrdU positive cells [%]

Sub-G1 peak (PI staining) [%]

CDKN1A mRNA expression norm.FC

SA-βGal positive cells [%]

HDF161

1.7

4.3

2.5

3.9

0.6

1.1

0.9

16.1

HDF85

1.1

4.1

2.8

0.7

4.8

1.3

1.7

1.0

HDF76

1.5

4.7

1.2

3.3

1.2

1.9

0.8

13.8

Abbreviations: Q = quiescent control, S = stress induced premature senescent, + = positive control – Staurosporin 300nM overnight, Y = proliferating control.

Table 1A. Detailed characteristics of major hallmarks of cellular senescence of the individual donors HDF161, HDF76 and HDF85 each senescent and quiescent control after 7 (D7) days post last H2O2 application.

Table 1A. Detailed characteristics of major hallmarks of cellular senescence of the individual donors HDF161, HDF76 and HDF85 each senescent and quiescent control after 7 (D7) days post last H₂O₂ application.