. 2018 May 19;10(5):1103–1132. doi: 10.18632/aging.101452

Table 1B. Detailed characteristics of major hallmarks of cellular senescence of the individual donors HDF161, HDF76 and HDF85 each senescent and quiescent control after 21 days (D21) post last H₂O₂ application.

3 week recovery (D21)

Donor

Total apoptotic cells (Annexin + PI positive) [%]

BrdU positive cells [%]

Sub-G1 peak (PI staining) [%]

CDKN1A mRNA expression norm.FC

SA-βGal positive cells [%]

HDF161

1.1

2.8

4.5

1.5

0.8

3.3

1.2

1.3

HDF85

0.7

5.4

3.8

0.4

1.2

3.2

1.1

1.5

HDF76

0.6

5.6

3.5

1.1

1.3

6.8

0.8

1.4

Abbreviations: Q = quiescent control, S = stress induced premature senescent, + = positive control – Staurosporin 300nM overnight, Y = proliferating control.

Table 1B. Detailed characteristics of major hallmarks of cellular senescence of the individual donors HDF161, HDF76 and HDF85 each senescent and quiescent control after 21 days (D21) post last H2O2 application.

Table 1B. Detailed characteristics of major hallmarks of cellular senescence of the individual donors HDF161, HDF76 and HDF85 each senescent and quiescent control after 21 days (D21) post last H₂O₂ application.