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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Anal Chem. 2017 Nov 7;89(22):12433–12440. doi: 10.1021/acs.analchem.7b03527

Figure 2.

Figure 2

(a) Schematic of the optical-fiber-based fluorometer. Fluorescence signal is collected by the parabolic mirror and detected by a USB 2000 spectrometer. Apparatus: ND, Neutral-density filter; L1 and L2, lenses; OAP, off-axis parabolic mirror; S, sample; F, optical fiber; and NF, notch filter. (b) Uncorrected emission curve of barcode reporter vs various concentrations. The peak is centered at ∼520 nm. (c) Fluorescence intensity vs barcode reporter concentrations shows linear dependence at the attomolar level. The limit of detection of using pure fluorescence probe for the system is ∼200 aM. Error bars indicate standard error of the mean.