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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Anal Chem. 2017 Nov 7;89(22):12433–12440. doi: 10.1021/acs.analchem.7b03527

Figure 3.

Figure 3

Optimization of solid-phase extraction process and assay. (a) Release efficiency of UV-cleavable probes vs release time with UV power ranging from ∼0.8 mW/cm2 (10 cm) to ∼1.6 mW/cm2 (0 cm). Error bars are standard error of the mean. (b) Capture efficiency results for 30 fM Ebola RNA (1× in buffer) as a function of incubation time (yellow box: 2 min; purple box: 10 min; green box: 20 min; red box: 40 min) compared with (1× in blood) and (10× in blood) target concentration procedure (1×: input 15 μL, release 15 μL; 10×: input 150 μL, release 15 μL). Error bars are confidence intervals. (c) Magnetic bead capture efficiency results as a function of incubation time for Ebola RNA-probe A-reporter tag-UV cleavable probe in blood. Error bars are standard error of the mean.