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. Author manuscript; available in PMC: 2018 Jun 6.
Published in final edited form as: Immunobiology. 2017 Jul 11;222(11):979–988. doi: 10.1016/j.imbio.2017.07.002

Fig. 5.

Fig. 5

Effect of SLFN14 knockdown on antiviral responses (A) RAW 264.7 cells were treated with either control siRNA or SLFN14 siRNA. After 24 h transfection, cells were mock infected or infected with A/PR8 or PR8/delNS1 at a multiplicity of infection of 1. The knockdown efficiency of SLFN14 siRNA was determined by measuring the expression levels of SLFN14 mRNA. (B) The effect of SLFN14 knockdown on IP-10 and IFN-β gene expression was also measured by realtime qRT-PCR. (C) Cells were pre-treated with recombinant IFN-β or transfected with either control siRNA or SLFN14 siRNA. Progeny viral titers were calculated and expressed as plaque-forming units (PFU)/mL. Data are shown as means ± SEM of three different experiments and are presented as the percentage relative to the control siRNA sample. Statistical analysis: *p < 0.05 compared to control siRNA-transfected cells.