Fig. 6.

SLFN14 promotes RIG-I-mediated signaling (A) HEK293T cells were transiently transfected with empty vector (EV), SLFN14, RIG-I, or SLFN14/RIG-I, along with reporter plasmids and a Renilla luciferase plasmid (internal control). Cells were stimulated with either DMSO control or poly I:C (5 µg/mL) Relative IFN-β luciferase activity is shown as fold induction over DMSO control. A representative of three independent experiments is presented. (B) Plasmids expressing RIG-I, SLFN14 (SL14) and influenza NS1 were transfected into HEK293T cells, together with the IFN-β reporter plasmids and Renilla luciferase plasmid (internal control). After 24 h, luciferase activity was measured. (C) Knockdown of RIG-I was performed with siRNA transfection. mRNA expression of SLFN14, RIG-I, MxA, IP-10, ISG15, and IFN-β was measured by RT-PCR.