Table 1.
Descriptive Measures Across Vitamin D Adjusted Chow Groups
| Measurement | Deficient Vitamin D (DD) (0 IU/g) Ca = 0.95%* | Insufficient Vitamin D (ID) (2.25 IU/g) Ca = 0.95%* | Sufficient Vitamin D Control (CD) (5 IU/g) Ca = 0.95%* | Hyper-Vitamin D (HD) (40 IU/g) Ca = 0.94%* | P-Value |
|---|---|---|---|---|---|
| Serum Levels | |||||
| Serum Vitamin D (ng/mL) srg** | 9.26 ± 1.61 | 20.64 ± 3.27 | 28.30 ± 4.57 | 101.49 ± 17.27 | p<0.0001 |
| Serum Vitamin D (ng/mL) sac** | 6.25 ± 1.02 | 15.58 ±5.04 | 19.59 ± 5.64 | 88.44 ± 9.91 | p<0.0001 |
| Total Calcium (mg/dL) sac | 11.69 ± 1.20 | 11.78 ± 0.49 | 11.48 ± 0.89 | 12.86 ± 0.76 | p<0.001 |
| Free Calcium (mg/dL) sac | 6.59 ± 0.85 | 6.83 ± 0.81 | 6.84 ± 1.07 | 7.11 ± 1.09 | NS |
| Bound Calcium (mg/dL) sac | 5.10 ± 1.74 | 4.95 ± 0.72 | 4.65 ± 1.29 | 5.75 ± 1.15 | NS (p=0.06) |
Values given as mean ± SD, NS = Not Significant
% Calcium by weight of rat chow diet
Repeated measures SAS-Mixed model yielded significant differences within-subjects effect of pre-surgery (4 wks) vs. sac (16 wks) serum vitamin D (P<0.0001), a main grouping effect (P<0.0001), and interaction effect p<0.05. Serum Vitamin D was significantly decreased at sacrifice (16wks) compared to pre-surgery (4wks) – especially for HD: where serum vitamin D was significantly decreased to 88.44 (16wks) vs. 101.49 (4wks) (P<0.001), and for CD: 19.59 (16wks) vs. 28.30 (4wks) (p<0.05) (Tukey-Kramer/Bonferroni).
Total Calcium sac: R2 = 0.27 P<0.001, contrast HD > CD, ID, DD, (p<0.05)
NS, Bound Calcium sac: SAS-GLM R2 = 0.07, p<0.069; contrast HD > CD (p<0.05)